Bio-Safety Cabinets (BSC) Classification

 

ClassProtection TargetAirflow DesignApplicationsRemarks
Class I✅ User
✅ Environment
❌ Product
Front air inlet → HEPA filter → air exhaust

Low-risk pathogen handling and simple sample manipulation

For example: Microbiological testing of non-volatile, non-toxic, or non-radioactive substances

Simple structure, similar to a Fume Hood
Class II A2✅ User
✅ Product
✅ Environment

70% Re-circulated air after filtered through HEPA

30% air exhaust after filtered through HEPA

Risk Group I ~ III pathogenic microorganisms

For example: Cell culture, blood samples, sterile drug preparation

Most common type, suitable for most medical/research labs
Class II B1✅ User
✅ Product
✅ Environment

70% air exhaust after filtered through HEPA

30% Re-circulated air

Risk Group I ~ III pathogenic microorganisms

For example: Experiments involving trace amounts of volatile, toxic, or radioactive substances

Has a larger air exhaust volume than A2 type Bio-Safety Cabinet

Need to connect with an external air duct

Class II B2✅ User
✅ Product
✅ Environment
100% air exhaust after filtered through HEPA

Risk Group I ~ III pathogenic microorganisms

For example: Experiments involving volatile, toxic, or radioactive substances

High cost, high energy consumption

Requires a dedicated air exhaust system

Class III

✅Highest Level
✅ User
✅ Product
✅ Environment

Fully enclosed and operates with attached gloves

Double HEPA filtration or HEPA filter with exhaust air incineration

Risk Group III ~ IV pathogenic microorganisms

For example: Handling high-risk pathogens (BSL-3/4), dangerous viruses, or bacteria

Also known as "Class III fully enclosed Bio-Safety Cabinet", it is the most stringent type of Bio-Safety Cabinet
 2025-08-28